Figure 1.

(A) Cartoon of the known functional regions of Rad1. (B) Alignment of N-terminal portions of Rad1 and human XPF. Residues 179 to 462 of Rad1 were aligned with XPF by Blast (NCBI). Conserved and similar residues are indicated. Conserved, charged residues were mutated to alanine, either individually or in clusters, by site-directed mutagenesis. Mutated residues are indicated in color. Residues mutated as a group are shown in the same color. Ten mutations were created in RAD1, spanning residues 203 through 456.