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. 2018 Apr 17;46(10):5139–5158. doi: 10.1093/nar/gky273

Figure 6.

Figure 6.

Cellular pyrophosphatases DOM3Z and DUSP11 limit both miR-122-dependent and miR-122-independent viral RNA accumulation. Huh-7.5 cells were electroporated with siDOM3Z (A–E) or siDUSP11 (F–J) or siControl (siCon) at day –2 and at day 0 cells were electroporated again with the indicated siRNA, and either (A, F) wild-type or GNN FL HCV RNA, and a firefly luciferase control mRNA or (B, G) S1+S2p3 SGR or S1+S2p3 GND SGR, a Renilla luciferase control mRNA, and miR-122p3 (miR-122-dependent) or miCon (miR-122-independent). Replication was measured by evaluating luciferase production at the indicated timepoints. (C, H) Western blot showing knockdown efficiency using antibodies against DOM3Z, DUSP11 and β-actin. Percent knockdown ± standard deviation relative to siCon-treated is indicated. (D, I) Northern blot analysis of FL HCV RNA accumulation during DOM3Z or DUSP11 knockdown at day 3. (E, J) Densitometry quantification of northern blot analysis of FL HCV RNA accumulation normalized to siCon. All data are representative of at least three independent experiments and statistical significance was determined by paired parametric t test.

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