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. 2018 Mar 27;46(10):5029–5049. doi: 10.1093/nar/gky227

Figure 10.

Figure 10.

(A) RT-qPCR for CPSF30 mRNA on total RNA extracted from C33A2, C33A6 or the parental C33A cells transfected with siRNAs to CPSF30 compared to the same cell lines transfected with scrambled siRNAs (scr). (B) sLuc activity induced by transfection of C33A2 and C33A6 cells with siRNAs to CPSF30 compared to cells transfected with scrambled siRNAs (scr). (C) Western blotting for influenza virus NS1 protein on extracts from C33A cells transfected with expression plasmids for influenza A virus H5N1 or H1N1–1918 NS1 protein. (D) sLuc activity at various time points after transfection of C33A2 and C33A6 cells with empty vector or expression plasmid for influenza A virus H1N1–1918 NS1 protein. (E) CAT protein levels produced from HPV16 subgenomic reporter plasmid pBELCAT (see Supplementary Figure S6B) transfected with plasmid pH5N1Gs that produces an influenza virus H5N1 NS1 protein that binds CPSF30, or with plasmid pH5N1Hn that produces an influenza virus H5N1 NS1 protein that cannot bind CPSF30. (F) Western blot for H5N1 NS1 protein in the same cell extracts that were analysed for CAT protein levels in (E).