Figure 11.

ChIP analyses on HPV16-positive tonsillar cancer cell line HN26 that contains episomal HPV16 DNA (unpublished results). Melphalan treated HN26 cells were subjected to ChIP analysis using antibodies to DDR factors BRCA1 and BLAF1 (A) and RNA binding proteins U2A65 and hnRNP C (B). Primers and antibodies are listed in Supplementary Tables S3 and 4, respectively, and the location in the HPV16 genome of the PCR primers is shown in Supplementary Figure S5. Mean values with standard deviations of the amount of immunoprecipitated DNA compared to DNA from DMSO-treated cells are displayed. The q-PCR values obtained for each primer pair with DNA extracted from DMSO-treated HN26 cells were set to 1 to correct for differences between different ChIP extracts. Chip extracts were prepared from C33A2 cells treated with melphalan for the indicated time-periods.