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. 2018 Jun 11;18(3):15. doi: 10.1093/jisesa/iey059

Table 1.

Primers used in this study

Primer name Primer sequence from 5′ to 3′ Use
Hsp70-F ATYATCGCCAAYGAYCARGG Degenerate PCR
Hsp70-R ATGACRCCACCRGCMGTYTC Degenerate PCR
Hsp90-F HTBGGCCGNGGHACCAAGAT Degenerate PCR
Hsp90-R GCYTGSGCYTTCATKATRCG Degenerate PCR
Action-F RTHGCCCCHGARGARCAYCC Degenerate PCR
Action-R CCWCCGATCCAKACRGAGTA Degenerate PCR
UPM-Long CTAATACGACTCACTATAGGGC AAGCAGTGGTATCAACGCAGAGT RACE
UPM-short CTAATACGACTCACTATAGGGC RACE
NUP AAGCAGTGGTATCAACGCAGAGT RACE
AP-C AAGCAGTGGTATCAACGCAGAG TACGCGGGGGGGGGG RACE
Hsp70-3-inner CTACAAGGAGGAGGACGACAAGCAGC 3′RACE
Hsp70-3-outer TCGACTTCTACACCAAGGTGTCCCG 3′RACE
Hsp90-3-inner GGGTCTTGAGCTTCCTGAAGATGAAG 3′RACE
Hsp90-3-outer GACTGAGCCCATTGACGAGTATGTTG 3′RACE
Hsp70-5-inner CCTGCCTCTGGCTGTTGAAGTAG 5′RACE
Hsp70-5-outer ATCTTGGTCAGGACCATGGAGCTGAT 5′RACE
Hsp90-5-inner GGTCCGCAACCAGGTAGGCTGAGTAG 5′RACE
Hsp90-5-outer TCTCCACCAGCAGCTTGATGGGATAT 5′RACE
qHsp70-F TCTTCAACGGCAAGTCGCTC q PCR
qHsp70-R AACTTGGTCATCACGCCACC q PCR
qHsp90-F ACGCGAGGAAGATAAGGCCA q PCR
qHsp90-R ACGTTCCATGTTGGCAGTCC q PCR
qAction-F TGCTGCATCATCAAGCTCCC q PCR
qAction-R GTCTCGTGGATACCGCAAGC q PCR
fHsp70-F CGTGAACGAATCTAAGTGACAATGCC Full-length validation
fHsp70-R TTAGTCGACTTCTTCAACTGAGGGGC Full-length validation
fHsp90-F CTTTCTTCGTTCCTTGTGCTCCGTT Full-length validation
fHsp90-R TGTGTCTTTGGCGATGATGAGATGA Full-length validation