Table 1.
Primer name | Primer sequence from 5′ to 3′ | Use |
---|---|---|
Hsp70-F | ATYATCGCCAAYGAYCARGG | Degenerate PCR |
Hsp70-R | ATGACRCCACCRGCMGTYTC | Degenerate PCR |
Hsp90-F | HTBGGCCGNGGHACCAAGAT | Degenerate PCR |
Hsp90-R | GCYTGSGCYTTCATKATRCG | Degenerate PCR |
Action-F | RTHGCCCCHGARGARCAYCC | Degenerate PCR |
Action-R | CCWCCGATCCAKACRGAGTA | Degenerate PCR |
UPM-Long | CTAATACGACTCACTATAGGGC AAGCAGTGGTATCAACGCAGAGT | RACE |
UPM-short | CTAATACGACTCACTATAGGGC | RACE |
NUP | AAGCAGTGGTATCAACGCAGAGT | RACE |
AP-C | AAGCAGTGGTATCAACGCAGAG TACGCGGGGGGGGGG | RACE |
Hsp70-3-inner | CTACAAGGAGGAGGACGACAAGCAGC | 3′RACE |
Hsp70-3-outer | TCGACTTCTACACCAAGGTGTCCCG | 3′RACE |
Hsp90-3-inner | GGGTCTTGAGCTTCCTGAAGATGAAG | 3′RACE |
Hsp90-3-outer | GACTGAGCCCATTGACGAGTATGTTG | 3′RACE |
Hsp70-5-inner | CCTGCCTCTGGCTGTTGAAGTAG | 5′RACE |
Hsp70-5-outer | ATCTTGGTCAGGACCATGGAGCTGAT | 5′RACE |
Hsp90-5-inner | GGTCCGCAACCAGGTAGGCTGAGTAG | 5′RACE |
Hsp90-5-outer | TCTCCACCAGCAGCTTGATGGGATAT | 5′RACE |
qHsp70-F | TCTTCAACGGCAAGTCGCTC | q PCR |
qHsp70-R | AACTTGGTCATCACGCCACC | q PCR |
qHsp90-F | ACGCGAGGAAGATAAGGCCA | q PCR |
qHsp90-R | ACGTTCCATGTTGGCAGTCC | q PCR |
qAction-F | TGCTGCATCATCAAGCTCCC | q PCR |
qAction-R | GTCTCGTGGATACCGCAAGC | q PCR |
fHsp70-F | CGTGAACGAATCTAAGTGACAATGCC | Full-length validation |
fHsp70-R | TTAGTCGACTTCTTCAACTGAGGGGC | Full-length validation |
fHsp90-F | CTTTCTTCGTTCCTTGTGCTCCGTT | Full-length validation |
fHsp90-R | TGTGTCTTTGGCGATGATGAGATGA | Full-length validation |