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. 2018 Mar 14;46(10):4933–4949. doi: 10.1093/nar/gky193

Figure 2.

Figure 2.

Mbd2 downregulation is crucial for globin expression and terminal erythroid differentiation. (A) Immunoblot analysis of the Mbd2, Mbd3, and p66α expression in Mbd2 KD, Mbd3 KD and Mbd DKD cells during HMBA-induced differentiation of MEL cells. (B) Immunoblot analysis of the α- and β-globin expression in uninduced (d0) and in HMBA-induced (d3) Mbd2 KD and Mbd3 KD MEL cells (adapted from the time-course data in Supplementary Figure S2A). The values normalized to β-tubulin are shown as mean ± SEM; n = 2. (C) RT-qPCR analysis of the α-globin expression in Mbd2 KD cells. Values normalized to Gapdh are shown as mean ± SEM; n = 3. (D) RT-qPCR analysis of the expression of erythroid markers in uninduced (d0) and in HMBA-induced (d3) Mbd2 KD and Mbd3 KD MEL cells. n = 2. (E) Functional hemoglobin synthesis analysis by benzidine staining. Fraction of benzidine stain-positive cells was scored. Values are mean ± SEM; n = 4. Bar = 100 μm.