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. 2018 Apr 6;46(10):5050–5060. doi: 10.1093/nar/gky239

Figure 3.

Figure 3.

Role of XPC phosphorylation in recruitment of NER factors to the chromatin post-UVB irradiation. (A) Immunoblot analysis of XPC and GAPDH 30 min after UVB exposure (20 mJ/cm2) in XPCNull cells expressing pLenti-XPC WT or mutant S94A. (B) Quantification of ratio of ubiquitinated/total XPC fraction from the western blots in (A). *P < 0.05, compared with WT, Student’s t-test. (C) Immunoblot analysis of XPC, XPB, XPA and Histone H3 using chromatin-bound protein fractions from XPCNull cells expressing pLenti-XPC WT or mutant constructs S892A or S94A, at the indicated times post-UVB exposure (20 mJ/cm2). (D) Quantification of ratio of ubiquitinated/total XPC fraction from the western blots in (C). *P < 0.05, **P ≤ 0.01; compared with WT, Student’s t-test. The results were obtained from three independent experiments.