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. Author manuscript; available in PMC: 2018 Jun 19.
Published in final edited form as: Biochemistry. 2018 Apr 17;57(18):2733–2743. doi: 10.1021/acs.biochem.8b00292

Figure 1. BrMT inhibits Kv1.4 channel activation.

Figure 1

(A) Kv1.4 current responses to indicated BrMT concentrations during voltage steps to from −100 mV to 0 mV. (B) Kv1.4 current responses. Colored lines correspond to same BrMT concentrations as panel A. Stippled lines are fits of Equation 2, in Supporting Information. Voltage step to from −100 mV to 0 mV. Vehicle τact = 1.72 ± 0.02, τinact = 67.3 ± 0.2; 1.5 μM τact = 2.54 ± 0.02, τinact = 136.6 ± 0.6; 3 μM τact = 3.17 ± 0.03, τinact = 181 ± 2; 6 μM τact = 4.2 ± 0.4, τinact = 300 ± 20. (C) Time constants of activation (filled circles) and inactivation (empty circles). Error bars indicate SEM. n = 9. Stippled lines are fits for Kd as in ref. 19. Kd from τact = 2.9 ± 1.0 μM, Kd from τinact = 1.6 ± 0.7 μM.