Figure 3.

Illustration of a microfluidic model recreating the biophysical microenvironment for tumor cell invasion studies. ECM or tumor cell embedded ECM (pink) is introduced into three wall-less channels confined by contact lines (yellow rectangular, not to scaled). Blood vessel (red) and lymphatic vessel (green) are formed by growing a layer of blood and lymphatic EC cells respectively. Blood and lymph flows are introduced into two vascular vessels (direction marked by x), and interstitial flow is introduced horizontally (direction marked by an arrow).