(A) Pie chart showing SLUG mRNA expression in breast cancer subtypes (PAM50 classification, cBioPortal). SLUG and TAZ expression was correlated using the same database (right graph).
(B and C) SLUG expression was depleted using shRNAs (shSLUG-1 and shSLUG-2) in SUM-1315, MDA-435, and MDA-231 cells, and nuclear fractions were blotted for SLUG and TAZ (B). The same cells were used to quantify TAZ target genes using qPCR (C).
(D) SKBR3 cells were transfected with an empty vector or a construct expressing SLUG, and nuclear fractions were blotted for SLUG and TAZ. Total RNA extracted from the same cells was used to quantify TAZ target genes by qPCR.
(E) Control and IMP3-depleted SUM-1315 cells were transiently transfected with an empty vector (EV) or SLUG-expressing construct. Total RNA was extracted and used to quantify TAZ target genes by qPCR.
(F) Total cell extracts from control and SLUG-depleted SUM-1315 and MDA-231 cells were blotted for pTAZ (S89) and LATS1 (T1079). p ≤ 0.05.
Relevant data are shown as ± SE.