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. Author manuscript; available in PMC: 2018 Jun 19.
Published in final edited form as: Cell Rep. 2018 May 29;23(9):2559–2567. doi: 10.1016/j.celrep.2018.04.113

Figure 4. WNT5B Mediates SLUG Transcription.

Figure 4

(A) Control and IMP3-depleted HEK293FT and MDA-435 cells were transfected with a reporter construct bearing a 4-kb SLUG promoter upstream of firefly luciferase, and they were assayed for luciferase activity (RLU) 24 hr post-transfection.

(B and C) Nuclear extracts from control and WNT5B-depleted SUM-1315 and MDA-231 cells were blotted for SLUG protein, and total RNA extracted from these cells was used to quantify SLUG mRNA (B) and TAZ target genes by qPCR (C).

(D) Control and IMP3-depleted MDA-435 cells (shIMP3-1 and shIMP3-2) were transiently transfected with an empty vector or WNT5B-expressing construct. Total RNA was extracted and used to quantify SLUG and WNT5B mRNA by qPCR.

(E) Control and IMP3-depleted HEK293FT cells were transfected with an empty vector (EV) or a construct expressing WNT5B. These cells were re-transfected 24 hr later with a SLUG-luciferase reporter construct and assayed for luciferase activity after another 24 hr.

(F) Expression of WNT5B and SLUG mRNA was correlated using cBioportal. The correlation coefficient (r) was estimated using Pearson’s correlation. *p ≤ 0.05.

Relevant data are shown as ± SE.