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. 2018 Jun 19;13(6):e0199356. doi: 10.1371/journal.pone.0199356

Table 4. Comprehensive stability ranking of the candidate reference genes.

Method 1 2 3 4 5 6 7 8 9
Ranking order for the cold experimental set (Better—Good—Average)
geNorm POB1/UFD2 UK552 HMT GAPDH ACT5 SuTub E2.299 TUB250
NormFinder POB1 UK552 UFD2 E2.299 AKB 60S897 TUB250 HMT SuTub
BestKeeper TUB250 AKB SuTub ARP9 UFD2 SPT POB1 E3.528 EF1.278
Comprehensive ranking POB1 UFD2 TUB250 UK552 AKB SuTub HMT E2.299 GAPDH
Ranking order for the drought experimental set (Better—Good—Average)
geNorm tIF AKB POB1 E2.299 HMT ACT5 GAPDH UK552 SPT
NormFinder POB1 tIF E2.299 AKB GAPDH UK552 SPT ACT5 HMT
BestKeeper POB1 ARP9 AKB SuTub GAPDH ACT5 E2.299 60S897 HMT
Comprehensive ranking POB1 AKB tIF E2.299 GAPDH ARP9 ACT5 HMT UK552
Ranking order for the WGF experimental set (Better—Good—Average)
geNorm E2.299 AKB UFD2 GAPDH HIS TUB250 tIF SPT POB1
NormFinder POB1 UFD2 E2.299 AKB HIS ARP9 GAPDH HMT tIF
BestKeeper UFD2 SPT GAPDH 60S897 HMT POB1 E3.528 EF1.278 AKB
Comprehensive ranking UFD2 AKB E2.299 POB1 GAPDH SPT HIS HMT TUB250
Ranking order for the total experimental set (Better—Good—Average)
geNorm POB1 AKB UFD2 E2.299 ARP9 TUB250 60S897 tIF HMT
NormFinder E2.299 UFD2 POB1 AKB tIF TUB250 ARP9 HMT 60S897
BestKeeper HMT AKB POB1 ARP9 E3.528 E2.299 UFD2 UK552 ACT5
Comprehensive ranking POB1 AKB E2.299 UFD2 HMT ARP9 TUB250 E3.528 tIF
Method 10 11 12 13 14 15 16 17
Ranking order for the cold experimental set (Better—Good—Average)
geNorm AKB 60S897 SPT tIF ARP9 E3.528 HIS EF1.278
NormFinder ACT5 GAPDH SPT tIF ARP9 E3.528 HIS EF1.278
BestKeeper HMT GAPDH E2.299 60S897 ACT5 UK552 HIS tIF
Comprehensive ranking ARP9 ACT5 60S897 SPT E3.528 EF1.278 tIF HIS
Ranking order for the drought experimental set (Better—Good—Average)
geNorm E3.528 HIS ARP9 EF1.278 UFD2 60S897 SuTub TUB250
NormFinder HIS E3.528 ARP9 EF1.278 UFD2 SuTub 60S897 TUB250
BestKeeper UFD2 EF1.278 UK552 TUB250 HIS SPT E3.528 tIF
Comprehensive ranking SPT SuTub HIS E3.528 EF1.278 60S897 UFD2 TUB250
Ranking order for the WGF experimental set (Better—Good—Average)
geNorm ARP9 HMT E3.528 60S897 UK552 EF1.278 ACT5 SuTub
NormFinder E3.528 TUB250 SPT UK552 60S897 EF1.278 ACT5 SuTub
BestKeeper TUB250 HIS ARP9 SuTub E2.299 UK552 tIF ACT5
Comprehensive ranking ARP9 60S897 E3.528 tIF EF1.278 UK552 SuTub ACT5
Ranking order for the total experimental set (Better—Good—Average)
geNorm SPT E3.528 ACT5 EF1.278 SuTub UK552 HIS GAPDH
NormFinder E3.528 ACT5 EF1.278 SPT SuTub HIS UK552 GAPDH
BestKeeper TUB250 SPT SuTub 60S897 GAPDH EF1.278 HIS tIF
Comprehensive ranking 60S897 ACT5 SPT UK552 EF1.278 SuTub HIS GAPDH