Table 1. NiV assay comparison.
| Virus | Virus Stock | Assay | Ct Values | Mean Viral RNA copies/mL | Coefficient of Variation |
|---|---|---|---|---|---|
| NiV-M |
Daughter A | NiV-M Assay | 22.8, 24.1, 22.6 | 7.87 x 1010 | 0.35 |
| NiV-M-F Assay | 14.8, 15.2, 15.0 | 3.87 x 1012 | 0.15 | ||
| NiV-M-G Assay | 15.1, 15.0, 15.2 | 3.72 x 1012 | 0.26 | ||
| Daughter B | NiV-M Assay | 23.0, 23.0, 22.5 | 8.99 x 1010 | 0.10 | |
| NiV-M-F Assay | 15.3, 15.2, 15.3 | 3.43 x 1012 | 0.04 | ||
| NiV-M-G Assay | 16.0, 15.7, 15.9 | 2.57 x 1012 | 0.03 | ||
| Daughter C | NiV-M Assay | 20.8, 21.9, 20.81 | 2.03 x1011 | 0.05 | |
| NiV-M-F Assay | 12.9, 12.7, 12.8 | 1.13 x 1013 | 0.09 | ||
| NiV-M-G Assay | 13.5, 13.5, 13.3 | 8.32 x 1012 | 0.04 | ||
| NiV-B | Daughter A | NiV-B Assay | 21.5, 21.4, 21.5 | 3.06 x 1010 | 0.03 |
| NiV-B-F Assay | 15.4, 15.5, 15.5 | 1.01 x 1012 | 0.05 | ||
| NiV-B-G Assay | 15.5, 15.6, 15.5 | 9.67 x 1011 | 0.05 | ||
| Daughter B | NiV-B Assay | 21.9, 21.8, 21.9 | 2.42 x 1010 | 0.04 | |
| NiV-B-F Assay | 15.8, 15.9, 15.9 | 8.02 x 1011 | 0.05 | ||
| NiV-B-G Assay | 16.0, 15.9, 15.9 | 7.85 x 1011 | 0.03 | ||
| Daughter C | NiV-B Assay | 22.6, 22.7, 22.5 | 1.59 x 1010 | 0.07 | |
| NiV-B-F Assay | 16.5, 16.6, 16.5 | 5.44 x 1011 | 0.03 | ||
| NiV-B-G Assay | 16.5, 16.6, 16.6 | 5.45 x 1011 | 0.03 |
NiV-M and NiV-B was passaged 3 consecutive times and a qRT-PCR was performed on each sample in triplicate using the assay that targets the F/G intergenic region, the F coding region or the G coding region. The coefficient of variation of the mean viral RNA copies/mL was calculated for each daughter sample analyzed.