Table 2. Calculation of NiV-M genome to PFU ratio.

Target titer (PFU/mL)	Unadjusted qRT-PCR (genomes/mL)	qRT-PCR dilution factor	Genomes/mL	PFU/mL*	Genome:PFU Ratio	Mean Genome:PFU Ratio	Coefficient of Variation
1.00 x 106	1.94 x 106	800	1.55 x 109	1.50 x 106	1.04 x 103	1.04 x 103	0.189
1.00 x 105	2.03 x 105	800	1.62 x 108	1.24 x 105	1.31 x 103
1.00 x 104	1.72 x 104	800	1.37 x 107	1.40 x 104	9.81 x 102
1.00 x 103	1.43 x 103	800	1.14 x 106	1.36 x 103	8.40 x 102

PFU, plaque forming unit; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction

All assays were performed in triplicate for each sample. The coefficient of variation of the mean genome:PFU ratio was calculated from using the genome:PFU ratios from the individual target titer samples.

*Based on back titration of assayed stock