Table 2. Calculation of NiV-M genome to PFU ratio.
Target titer (PFU/mL) | Unadjusted qRT-PCR (genomes/mL) | qRT-PCR dilution factor | Genomes/mL | PFU/mL* | Genome:PFU Ratio | Mean Genome:PFU Ratio | Coefficient of Variation |
---|---|---|---|---|---|---|---|
1.00 x 106 | 1.94 x 106 | 800 | 1.55 x 109 | 1.50 x 106 | 1.04 x 103 | 1.04 x 103 | 0.189 |
1.00 x 105 | 2.03 x 105 | 800 | 1.62 x 108 | 1.24 x 105 | 1.31 x 103 | ||
1.00 x 104 | 1.72 x 104 | 800 | 1.37 x 107 | 1.40 x 104 | 9.81 x 102 | ||
1.00 x 103 | 1.43 x 103 | 800 | 1.14 x 106 | 1.36 x 103 | 8.40 x 102 |
PFU, plaque forming unit; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction
All assays were performed in triplicate for each sample. The coefficient of variation of the mean genome:PFU ratio was calculated from using the genome:PFU ratios from the individual target titer samples.
*Based on back titration of assayed stock