Table 4. Passaged NiV genome to PFU ratio.
| Virus | Virus Stock | Calculated Titer | Calculated Viral RNA copies/mL | Genome:PFU ratio | Mean Genome:PFU ratio | Coefficient of Variation |
|---|---|---|---|---|---|---|
| NiV-M | Passage 1 | 3.89 x 107 | 7.87 x 1010 | 2.02 x 103: 1 | 1.40 x 103: 1 | 0.417 |
| Passage 2 | 4.33 x 107 | 8.61 x 1010 | 1.99 x 103: 1 | |||
| Passage 3 | 9.22 x 107 | 1.17 x 1011 | 1.27 x 103: 1 | |||
| Passage 4 | 1.27 x 108 | 8.99 x 1010 | 7.08 x 102: 1 | |||
| Passage 5 | 1.97 x 108 | 2.03 x 1011 | 1.03 x 103: 1 | |||
| NiV-B | Passage 1 | 1.97 x 107 | 3.06 x 1010 | 1.55 x 103: 1 | 1.53 x 103: 1 | 0.212 |
| Passage 2 | 1.28 x 107 | 2.42 x 1010 | 1.89 x 103: 1 | |||
| Passage 3 | 1.42 x 107 | 1.59 x 1010 | 1.12 x 103: 1 | |||
| Passage 4 | 1.78 x 107 | 3.20 x 1010 | 1.80 x 103: 1 | |||
| Passage 5 | 2.36 x 107 | 3.06 x 1010 | 1.30 x 103: 1 |
NiV-M and NiV-B were passaged 5 consecutive times and a titer and genome concentration was calculated from each sample. A mean genome:PFU ratio and the coefficient of variation was calculated from the 5 individual samples. All assays were performed in triplicate.