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. 2018 Jun 19;13(6):e0199534. doi: 10.1371/journal.pone.0199534

Table 4. Passaged NiV genome to PFU ratio.

Virus Virus Stock Calculated Titer Calculated Viral RNA copies/mL Genome:PFU ratio Mean Genome:PFU ratio Coefficient of Variation
NiV-M Passage 1 3.89 x 107 7.87 x 1010 2.02 x 103: 1 1.40 x 103: 1 0.417
Passage 2 4.33 x 107 8.61 x 1010 1.99 x 103: 1
Passage 3 9.22 x 107 1.17 x 1011 1.27 x 103: 1
Passage 4 1.27 x 108 8.99 x 1010 7.08 x 102: 1
Passage 5 1.97 x 108 2.03 x 1011 1.03 x 103: 1
NiV-B Passage 1 1.97 x 107 3.06 x 1010 1.55 x 103: 1 1.53 x 103: 1 0.212
Passage 2 1.28 x 107 2.42 x 1010 1.89 x 103: 1
Passage 3 1.42 x 107 1.59 x 1010 1.12 x 103: 1
Passage 4 1.78 x 107 3.20 x 1010 1.80 x 103: 1
Passage 5 2.36 x 107 3.06 x 1010 1.30 x 103: 1

NiV-M and NiV-B were passaged 5 consecutive times and a titer and genome concentration was calculated from each sample. A mean genome:PFU ratio and the coefficient of variation was calculated from the 5 individual samples. All assays were performed in triplicate.