Table 1. List of SNPs/Indels identified for each selection process, attributed to selection under low phosphate conditions.
Each transcript was found to harbor single or multiple SNPs in the coding region.
| Transcripts with SNP/Indels | Ref. Position |
SNP/ Indel |
Coverage depth (×) | Q-value | SNP % | |
|---|---|---|---|---|---|---|
| Week 6 | Cpro00002392—Auxenochlorella protothecoides strain 0710 sucrose phosphate synthase | 431 | T>C | 51 | 60 | 100% |
| Cpro00002508—Chlorella protothecoides mRNA for putative MO25 protein (dee76 gene) | 209 | G>A | 60 | 60 | 98% | |
| Cpro00007487—Chlorella protothecoides partial mRNA for putative extensin-like protein (dee189 gene) | 134 | G>del | 21 | 60 | 95% | |
| Cpro00009885—Auxenochlorella protothecoides strain 0710 NADPH-dependent FMN reductase mRNA, partial cds | 904 | TGT > del | 281 | 60 | 74% | |
| Cpro00007215—Auxenochlorella protothecoides actin gene | 212 552 |
T>G|T A>C|A | 66 461 |
60 | 60% 50% | |
| Week 10 | Cpro00002508—Chlorella protothecoides mRNA for putative MO25 protein (dee76 gene) | 209 | G>A | 49 | 60 | 94% |
| Cpro00009885—Auxenochlorella protothecoides strain 0710 NADPH-dependent FMN reductase | 904 | TGT > del or TGT | 61 | 60 | 62% | |
| Cpro00003782—Chlorella protothecoides mRNA for putative MINE protein (dee22 gene) | 126 | T>G|T | 58 | 60 | 53% | |
| Cpro00007215—Auxenochlorella protothecoides actin | 212 552 |
T>G|T A>C|A | 53 304 |
60 | 53%, 47% | |
| Cpro00013040—Chlorella variabilis V-type H+ ATPase subunit A (CHLNCDRAFT 56410) | 20 | T>G|T | 24 | 60 | 46% | |
| >Week 14 | Cpro00004400—Chlorella variabilis ribulose-phosphate 3-epimerase mature form (CHLNCDRAFT 56033) | 141 159 120 162 |
T>C T>C C>G G>C |
41 31 43 30 |
60 60 60 58 |
100%100%98% 97% |
| Cpro00021357—Chlorella variabilis 40S ribosomal protein S5 (CHLNCDRAFT 37294) | 171 204 214 216 |
C>G C>G G>A G>C |
123 201 95 94 |
60 60 58 58 |
88% 93% 87% 86% |
|
| Cpro00007379—Chlorella variabilis ribosomal protein L19 (CHLNCDRAFT 33146) | 228 261 |
C>G G>C |
461 558 |
60 60 |
89% 87% |
|
| >Week 22 | Cpro00021357—Chlorella variabilis 40S ribosomal protein S5 (CHLNCDRAFT 37294) | 204 171 216 214 |
C>G C>G G>C G>A |
63 40 27 27 |
60 60 60 60 |
98% 98% 96% 96% |
| Cpro00007379—Chlorella variabilis ribosomal protein L19 (CHLNCDRAFT 33146) | 228 261 |
C>G G>C |
146 187 |
60 60 |
96% 95% |
Note: deletion is abbreviated as ‘del’.