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. 2018 Jun 19;13(6):e0199475. doi: 10.1371/journal.pone.0199475

Table 2. DNA oligo nucleotides used in this study.

Primer Primer sequence (5’ to 3’) Source
pSW4426T-MCS-F CTCAACGGGAATCCTGCTCTGCGAG [15]
pSW4426T-MCS-R ACTGCTTGGTGCCAGCCAATGAG [15]
pSW4426T-cat-F CGCCGGCCAGCCTCGCAGA This study
pSW4426T-oriT-R TTACGCCCCGCCCTGCCACTC This study
pBU246-MCS-F GTGCAGGTCGTAAATCACTGCATAA This study
pBU246-MCS-R CAGAGCAAGAGATTACGCGCAGACCA This study
ompU-up-ClaI-F ATATATATCGATATCACAGTCAATCAAAGTGACGGTT This study
ompU-up-OEX-R AGAAGTCGTAACGTAGGATCAGAGTTTTGTTCATCTTATAAGTCCTAA This study
ompU-down-OEX-F GAACAAAACTCTGATCCTACGTTACGACTTCTAATTTGTGACTTAAT This study
ompU-down-SpeI-R ATATATACTAGTACGCTGTTTAACATTCGTTGAGTGTAA This study
Sp/Sm-EcoRV-F ATATATGATATCAAAGGTCATTCAAAAGGTCATCCACCGGAT This study
Sp/Sm-EcoRV-R ATATATGATATCAAAGGTCATCCACCGGATCAGCTTAGTAAA This study
pACT3-Cm-up ACCTCTTACGTGCCGATCAACGTCTCAT This study
pACT3-Cm-down TATTGGTGCCCTTAAACGCCTGGTGCT This study
pSW4499-cat-F TTACGCCCCGCCCTGCCACTC This study
pSW4499-oriT-R CGCCGGCCAGCCTCGCAGA This study
Vcor-toxR-SD-EcoRI-F ATATATGAATTCTGATGAAATCTATACAACAATAATGGTCATATCCA This study
Vcor-toxR-BamHI-R ATATGGATCCCTATTGGCAAACTTTACTGAGGTCTTGCTG This study
Vcor-ompU-SD-EcoRI-F ATATATGAATTCGGACAGTAAATTAGGACTTATAAGATGAAC This study
Vcor-ompU-SalI-R ATATATGTCGACTTAGAAGTCGTAACGTAGACCTAGAGCT This study