Inferences of a) the DNA strand segregation probability and b) mutation rate per cell division are robust against wide ranges of the stem cell proliferation rate λ. If stem cells divide once per week this implies (Eq (3)) for the probability of DNA strand segregation in colon: p = 0.973 (0.892; 0.996), small intestine: p = 0.969 (0.877; 0.995), liver: p = 0.988 (0.952; 0.998), prefrontal cortex: p = 0.999 (0.997; 0.9999), hippocampal dentale gyrus: p = 0.999 (0.997; 0.9999), skin: p = 0.985 (0.94; 0.998). Numbers in brackets correspond to the range of the DNA strand segregation probabilities for stem cell replication rates between once per month and every day respectively. In contrast for neurons during early development we find: p = 0.876 (0.67; 0.96) if cells divide every 48h (number in brackets correspond to cell divisions once per week and twice a day respectively). Based on Eq (4) we find for the in vivo mutation rate per base pair per cell division in colon: μ = 4.37 (4.27; 4.77) × 10−9, small intestine: μ = 3.54 (3.45; 3.91) × 10−9, liver: μ = 8.48 (8.39; 8.8) × 10−9, prefrontal cortex: μ = 7.68 (7.67; 7.7) × 10−8, hippocampal dentale gyrus: μ = 1.14 (1.14; 1.15) × 10−7, neurons during early development: μ = 1.23 (1.02; 1.47) × 10−8 and skin: μ = 1.57 (1.56; 1.65) × 10−7.