Figure 13.

MTH1 inhibitor interferes with cell migration and invasion: (A) The inhibitory effect of TH588 on KNS42 migration was manifested in the wound-healing assay as a difference in the width of the scratch between control and TH588 treated cells. Control untreated cells at time 0 (a) and four days later (b). TH588 treated cells at time 0 (c) and four days later (d). Bar, 400 μM. (B) TH588 inhibits the capacity of KNS24 for invasion: Control (a,c) and TH588 treated cells (b,d). Bar, 100 μM. (C) The extent of TH588 inhibition of KNS42 migration (a) and invasion (b) are presented in the histograms. Differences between control and experimental was specific p<0.005. Numbers are mean ± S.E.M of the travelled distance in μM measured at 10 different fields (a) and of cells at the bottom side of the insert at the end of the experiment counted at 5 different fields (b). (D) TH588 at 0.25 μM does not affect the clonogenic survival of KNS42.