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. 2018 Jan 11;9(44):27574–27584. doi: 10.18632/oncotarget.24164

Figure 2. Coexpression detection of CD300A, PECAM1, and ADCY7 in AML cell line U937.

Figure 2

(A) The knockdown efficiencies of CD300A, PECAM1, and ADCY7 were detected by RT-PCR, which indicated the expression of these three genes was significantly decreased in each knockdown group. (C) ADCY7 and PECAM1 were significantly down-regulated in the CD300A knockdown group. (D) The expression of CD300A showed no significant difference between NC and ADCY7- groups, as well as PECAM1. (E) The expression of CD300A showed no significant difference between NC and PECAM1- groups, as well as ADCY7. (B, F–H) Western blot analysis was performed to detect the expression of these three genes in each group based on protein level. F, CD300A only showed a significant decrease in group CD300A-. G, ADCY7 showed a significant decrease in groups ADCY7- and CD300A-. H, PECAM1 showed significant decreases in groups PECAM1- and CD300A-. These results suggest that the knockdown of CD300A caused a decrease in expression levels of ADCY7 and PECAM1.