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. 2018 Feb 19;9(44):27321–27332. doi: 10.18632/oncotarget.24535

Figure 1. The ataxin-3 (AT3) rs8021276 polymorphism significantly correlated with AT3 expression and aflatoxin B1 (AFB1)-DNA adducts in the liver tissues with cirrhosis.

Figure 1

(A–D) The level of AT3 protein in liver tissue samples (n = 384) was tested by immunohistochemistry (IHC) and scored using the IHC scores of IRS system (see Material and Methods). Representative images show that different expression levels were observed in liver tissues from cases with different AT3 rs8021276 genotypes (scale bars: 50 mm) (A–C). The association between AT3 rs8021276 genotypes and AT3 protein levels was evaluated by spearman correlation test (D). E-F, Total RNAs and DNAs were extracted from fresh liver tissues with cirrhosis (n = 46), and use to test the levels of AT3 mRNA expression (E) and the amount of AFB1-DNA adducts (F) by TaqMan-PCR and comparative enzyme-linked immunosorbent assay. Data were analyzed using one-way ANOVA test (with Bonferroni correction). Abbreviations: LAE, low AT3 protein expression; MAE, medium AT3 protein expression; HAE, high AT3 protein expression.