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. 2018 Jun 8;9(44):27363–27379. doi: 10.18632/oncotarget.25221

Figure 1. Characterization of recombinant protein activity and selection of AGR2 binding peptides by mRNA display.

Figure 1

(A) Homology between amino acid sequences from NCBI database. Homo sapiens: AGR2 CCDS5364.1 and AGR3 CCDS5365, were aligned by ClustalW. Asterisks indicate conserved amino-acids between the two proteins (65% identity). (B) Δ27 AGR2 enhances soft-agar colony formation. LNCaP and PC-3 cell lines were treated with recombinant AGR2 (100 ng/mL) for 72 hours. Formation of colonies was counted by microscopy. (C) Cell migration assay was performed for 48 hours by traditional Boyden Chamber method with recombinant Δ27 AGR2 and Δ27 AGR2-BAP. (D) Cartoon of the selection process for AGR2 binding peptides by mRNA display. All data represent at least three independent biological replicates. Asterisks indicate statistical significance with greater than 95% confidence (p<0.05) as evaluated with the students t-test.