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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Nature. 2018 Apr 25;557(7703):62–67. doi: 10.1038/s41586-018-0058-6

Extended Data Fig. 1. Reconstitution of GSDMA3 pores in vitro.

Extended Data Fig. 1

a, A general procedure for GSDMA3 pore reconstitution. b, Size-exclusion chromatography of GSDMA3 pores extracted from liposomes (top) and Coomassie blue-stained SDS–PAGE of the collected fractions. c, Representative negative-stain electron microscopy images of GSDMA3. d, Representative negative-stain electron microscopy images of human GSDMD pores with double-rimmed side views. e, f, Representative cryo-EM images of GSDMA3 pores with double-rimmed side views (e) and HgCl2-treated GSDMA3 pores (f). Scale bar, 50 nm (c–f). All results were confirmed at least three times as technical replicates.