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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Nature. 2018 Apr 25;557(7703):62–67. doi: 10.1038/s41586-018-0058-6

Extended Data Fig. 2. Cryo-EM analysis of double- and single-ring GSDMA3 pores.

Extended Data Fig. 2

a–c, Gold-standard FSC plots from two half-reconstructions refined separately in RELION for the 27-fold double-ring pore map (a), the 27-fold single-ring pore map (b) and the 28-fold single-ring pore map (c). Model-to-map correlations are shown for the 27-fold single-ring pore (b). d, e, Local resolution estimation generated by ResMap on the two half maps separately refined in a gold-standard procedure in RELION. Local resolutions are colour-coded on the densities. Highest resolution is observed at the β-barrel domain for both the single-ring pore (d) and the membrane-inserted ring of the double-ring pore (e). The globular domains and the additional ring exhibit relatively low resolution.