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. Author manuscript; available in PMC: 2018 Jun 19.
Published in final edited form as: Methods Cell Biol. 2015 Sep 2;131:21–90. doi: 10.1016/bs.mcb.2015.07.001

Fig. 14.

Fig. 14

Images of neurofilaments acquired using EMCCD and sCMOS cameras compared to images acquired using a conventional CCD camera. Wide-field epifluorescence microscopy of cultured rat cortical neurons expressing GFP-NFM. Camera exposure time=100 milliseconds. A. An axonal neurofilament imaged with an Andor Neo sCMOS camera (circa 2013). A′. The same neurofilament imaged with a Photometrics CoolSNAP HQ camera (circa 2002). B. An axonal neurofilament imaged with an Andor Ultra 897 EMCCD camera (circa 2013). B′. The same neurofilament imaged with a Photometrics CoolSNAP HQ camera (circa 2002). Note that both the EMCCD and sCMOS cameras result in a significant improvement in image contrast due to the higher signal-to-noise ratio. The cameras have different pixel sizes so the images are different sizes; the EMCCD image (160 nm pixels) was enlarged two-fold to make it similar to the sCMOS (65 nm pixels) and CCD (64.5 nm pixels) images. To avoid resampling, the images were not resized further. Scale bars = 4 μm.