Table 1.

Composition of SCG neuron culture media. The L-15 culture medium is maintained at atmospheric CO₂ and the DMEM/F12 culture medium is maintained at 5% CO₂. DMEM/F12 is a 1:1 (v/v) mixture of Dulbecco’s Modified Eagle and Ham’s F-12 nutrient media. 100 μg/ml bovine transferrin can be substituted with 20 μg/ml rat transferrin.

L-15 CULTURE MEDIUM
Component	Source
Leibovitz’s L-15 medium, phenol red-free	Gibco Life Technologies
10% (v/v) adult rat serum	Harlan Laboratories
0.6% (w/v) D-glucose (33.3 mM)	Sigma
0.29 mg/ml L-glutamine (2 mM)	Sigma
0.5% (w/v) Methocel™ (F4M Premium grade)	Dow Chemical Company
50 ng/ml NGF, 2.5S subunit	BD Biosciences

DMEM/F12 CULTURE MEDIUM
Component	Source
DMEM/F12 medium, phenol red-free	Gibco Life Technologies
100 μg/ml apo-transferrin, bovine	Sigma
10 μg/ml insulin, bovine	Sigma
5 ng/ml sodium selenite (30 nM)	Sigma
0.2 mg/ml L-glutamine (1.4 mM)	Sigma
0.5% (w/v) bovine serum albumin, Fraction V	EMD Millipore
0.5% (w/v) Methocel™ (F4M Premium grade)	Dow Chemical Company
50 ng/ml NGF, 2.5S subunit	BD Biosciences