. Author manuscript; available in PMC: 2018 Jun 19.

Published in final edited form as: Methods Cell Biol. 2015 Sep 2;131:21–90. doi: 10.1016/bs.mcb.2015.07.001

Table 2.

Composition of cortical neuron and glia culture media. These media are maintained at 5% CO₂.

Glia MEM CULTURE MEDIUM
Component	Source
Minimum Essential Medium (MEM)	Gibco Life Technologies
10% (v/v) horse serum	Gibco Life Technologies
0.7% (w/v) D-glucose (39 mM)	Sigma
5 μg/ml gentamicin	Sigma

NbActiv4^™ PLATING MEDIUM
Component	Source
NbActiv4^™ medium	BrainBits
5% (v/v) fetal bovine serum (FBS)	Hyclone, GE Healthcare Life Sciences
5 μg/ml gentamicin	Sigma

NbActiv4^™ CULTURE MEDIUM
Component	Source
NbActiv4^™ medium	BrainBits
37.5 mM NaCl (included ~1 week after plating)	Sigma