Figure 3. Half-lives of proteins with different cellular locations and functions.

(A) Half-life distribution of proteins in different cellular compartments (LocTree annotation; score ≥50) and of synaptic proteins (extracted based on UniProt GO annotation). Number of protein groups assigned to each compartment is given in brackets. Mitochondrial proteins are significantly longer-lived compared to the complete set of quantified half-lives. Except in mitochondria, membrane proteins tend to be shorter-lived compared to soluble proteins in each compartment. Selected GO terms for cellular component (B) as well as biological process, molecular function and Panther protein class (C) that are over-represented (p-value<0.05) in distinct half-life segments. The complete set of over-represented GO terms and the full name of abbreviated GO terms (marked with *) are given in Figure 3—source data 2.

Figure 3—figure supplement 1. N-terminal sequence analysis.

N-terminal sequence analysis of protein groups that contain one protein. Initiator methionine is not considered. (A) No significant over-representation or under-representation of individual amino acids at the N-terminus (position 1; p<0.05 indicated by red line) of short-lived proteins (10% with shortest half-lives). All other protein groups were used as reference. (B) Same for long-lived proteins (10% with longest half-lives). (C) Percentage of proteins with a destabilizing amino acid (R, K, H, F, W, Y, I, L) at the N-terminus.