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. 2018 Jun 19;7:e34202. doi: 10.7554/eLife.34202

Figure 5. Protein half-lives within multi-protein complexes.

(A) Standard deviation (SD) for half-lives within multi-protein complexes compared to SD between randomly sampled proteins. (B) Structures of the spliceosome (PDB code: 5O9Z), (C) the ribosome (PDB code: 3J7R), (D) the nuclear pore complex (multiple PDB codes, see Materials and methods) and (E) the ATP-synthase (PDB code: 5LQX) are color-coded according to the protein half-lives. For the nuclear pore complex (D) only one repeating unit is displayed. The whole complex is shown as an insert (bottom right). RNA molecules and proteins for which no half-life was determined are shown in grey. (F) Half-life distributions of the complex constituents and the corresponding color scales. (G) Relationship between the SD of complex members and complex assembly and disassembly dynamics. Average half-lives of complex members are indicated by the indicated color scale.

Figure 5—source data 1. Half-lives of protein complex constituents.
elife-34202-fig5-data1.xlsx (39.4KB, xlsx)
DOI: 10.7554/eLife.34202.017
Figure 5—source data 2. Half-lives of constituents of the ribosome, ATP-synthase, spliceosome and nuclear pore complex.
elife-34202-fig5-data2.xlsx (20.3KB, xlsx)
DOI: 10.7554/eLife.34202.018

Figure 5.

Figure 5—figure supplement 1. Correlation of half-life variability within protein complexes and complex size.

Figure 5—figure supplement 1.

Standard deviation of half-lives within 314 multi-protein complexes versus number of complex constituents for which a half-life was determined. No significant correlation was found (Pearson correlation = 0.01; p-value=0.84).