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. 2018 May 29;7:e33465. doi: 10.7554/eLife.33465

Figure 2. Mto1[NE] is localized to the cytoplasmic face of the nuclear pore complex.

Figure 2.

(A) Colocalization of Mto1[9A1-NE]-GFP and Nup146-3mCherry after nuclear pore complex (NPC) clustering in nup132∆ cells. For each cell, a single central Z-section is shown. (B) Immunoelectron microscopy of Mto1[9A1-NE]-GFP. Left panel shows cross-section of a single cell. NPCs can be seen as slightly electron-dense regions where inner and outer nuclear membranes meet. Yellow arrowheads indicate NPCs with Mto1[9A1-NE]-GFP on cytoplasmic face of NPC. White arrowheads indicate examples of unstained NPCs. Right panels show magnified examples from other cells. Blue and yellow dots indicate inner and outer nuclear membranes, respectively. N, nucleus; C, cytoplasm; m, mitochondria. Bars, 5 µm (A), 0.5 µm (B).