Table 1. Data for selected proteins from mass spectrometry comparison of GFP-Mto1[9A1-NE]-HTB and GFP-Mto1[9A1-bonsai]-HTB interactomes.
Peptide counts and label-free quantification (LFQ) values for selected proteins shown in Figure 1F. Data from two independent biological replicates are shown. Nsp1 and Nup82 are included as likely representative Nup146 interactors, based on homology to budding yeast (Belgareh et al., 1998). See also Supplementary file 3.
| Protein | Replicate 1 (E160307) | Replicate 2 (E161126) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Peptides from strain KS7611 | Peptides from strain KS8371 | LFQ intensity from strain KS7611 | LFQ intensity from strain KS8371 | LFQ ratio | Peptides from strain KS7611 | Peptides from strain KS8371 | LFQ intensity from strain KS7611 | LFQ intensity from strain KS8371 | LFQ ratio | |
| Alp7 | 17 | 3 | 2.3e8 | 7.5e6 | 30.5 | 14 | 5 | 1.9e8 | 3.7e7 | 5.2 |
| Crm1 | 20 | 5 | 2.7e8 | 1.7e7 | 15.6 | 18 | 5 | 2.7e8 | 2.0e7 | 13.7 |
| Mto1 | 59 | 46 | 2.8e11 | 2.3e11 | 1.2 | 52 | 43 | 2.8e11 | 2.5e11 | 1.1 |
| Mto2 | 14 | 20 | 6.6e9 | 5.1e9 | 1.3 | 16 | 22 | 6.7e9 | 5.8e9 | 1.2 |
| Nsp1 | 14 | 11 | 2.5e8 | 1.8e8 | 1.4 | 14 | 12 | 2.2e8 | 1.3e8 | 1.7 |
| Nup146 | 20 | 2 | 2.1e8 | 7.7e6 | 27.3 | 20 | 1 | 2.1e8 | NQ | NQ |
| Nup82 | 13 | 9 | 1.5e8 | 7.3e7 | 2.1 | 9 | 4 | 7.4e7 | 2.2e7 | 3.4 |
| Plo1 | 28 | 13 | 4.9e8 | 1.1e8 | 4.6 | 20 | 9 | 3.3e8 | 6.3e7 | 5.3 |
NQ = not quantified, because peptide count in the relevant sample was below threshold for quantification.