Fig. 4.

Cyclooxygenase-2 band migration was altered in the presence of the glycosylation inhibitor tunicamycin. A: representative Western blot of cyclooxygenase (COX-2) isoform levels and their band migration in human aortic vascular smooth muscle cells (HAoVSM) treated with vehicle (Veh; water-5% BSA), palmitate (Pal; 100 µM), palmitate (100 µM) plus tunicamycin (TN, 1 µg/ml), or tunicamycin for 18 h. B: analysis of COX-2 band intensity normalized to β-actin is summarized. Values are expressed as means ± SE. *P < 0.05 vs. Veh; #P < 0.05 vs. palmitate; n = 3/group.