Cyclooxygenase-2 band migration was altered in the presence of the glycosylation inhibitor, glucosamine-HCl. A: representative Western blot of cyclooxygenase (COX-2) levels and band migration in human coronary vascular smooth muscle cells (HCVSM). Cells were treated with vehicle (Veh; water-5% BSA), palmitate (100 µM), palmitate plus GS-HCl (1, 2.5, and 5 mM), or GS-HCl (1, 2.5, 5 mM) for 18 h. B: analysis of band intensity is summarized. COX-2 band intensity was normalized to β-actin (loading control). Values are expressed as means ± SE. *P < 0.05 vs. Veh; n = 3/group.