Table 2.
Quantitative real-time reverse transcription PCR to measure gene expression in the RA and LA
| Gene | LA ΔCT |
RA ΔCT |
||
|---|---|---|---|---|
| WT | KO | WT | KO | |
| Gnai1 | 6.75 ± 0.06 | 6.98 ± 0.05 | 8.46 ± 0.35 | 8.15 ± 0.09 |
| Gnai3 | 6.90 ± 0.18 | 6.65 ± 0.08 | 6.98 ± 0.12 | 7.42 ± 0.09 |
| Gnb1 | 4.76 ± 0.14 | 5.72 ± 0.25* | 5.21 ± 0.50 | 5.50 ± 0.07 |
| Gnb4 | 7.98 ± 0.18 | 7.72 ± 0.07 | 8.75 ± 0.18 | 8.60 ± 0.08 |
| Gng7 | 10.8 ± 0.14 | 11.5 ± 0.14 | 11.5 ± 0.51 | 11.7 ± 0.16 |
| Gng11 | 7.04 ± 0.11 | 7.49 ± 0.03* | 7.56 ± 0.21 | 7.43 ± 0.04 |
| Kcnj3 | 2.86 ± 0.14 | 2.30 ± 0.07 | 3.62 ± 0.18 | 3.00 ± 0.05 |
| Kcnj5 | 5.38 ± 0.12 | 4.86 ± 0.06* | 5.51 ± 0.24 | 4.94 ± 0.07* |
Values are means ± SE. Quantitative real-time reverse transcription PCR was performed as described in materials and methods for the genes indicated. Measurements were performed in triplicate from Gαi2−/− mice (n = 3 mice) and littermate controls (n = 3 mice). WT, wild type; KO, knockout.
*
P < 0.05 using one-way ANOVA.