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. 2001 Oct 16;98(22):12491–12496. doi: 10.1073/pnas.211291398

Figure 5.

Figure 5

Uptake of fluorescent Alexa 594-Tf by cultured rat yolk sac cells. (A) Uptake at 37°C of prebound Alexa 594-Tf (red) for 1, 5, or 10 min and immunostaining for cubilin using an Alexa 488-conjugated antibody (green). Note the initial colocalization of Tf and cubilin (1–5 min) and the subsequent vesicular accumulation of Tf in cubilin-negative structures (10 min). (B) Uptake at 37°C of prebound Alexa 594-Tf (red) for 5 min and immunostaining for TfR using an Alexa 488-conjugated antibody (green). Note the vesicular accumulation of Tf in TfR-negative cells.