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. 2018 Jan 3;314(5):R655–R666. doi: 10.1152/ajpregu.00405.2017

Fig. 5.

Fig. 5.

Expression of transient receptor potential vanilloid type 1 (TRPV1) in dorsal root ganglia (DRG; C7–8) of rats. Rats were treated with sham acupuncture, manual acupuncture (MA), or electroacupuncture (EA) after intrathecal injection of TRPV1 siRNA (+) or scrambled siRNA (−). A: examples of original Western blotting bands from one rat in each group. B: relative values of TRPV1/β-actin are shown in the bar graphs. C: percentage of TRPV1-labeled neurons in DRG of each group. Group data include: n = 24 DRG sections in four rats treated with TRPV1 siRNA + MA, and n = 18 DRG sections in three rats in each of four other groups, including sham acupuncture, scrambled siRNA + MA, scrambled siRNA + EA, and TRPV1 siRNA + EA. #P < 0.01, TRPV1 siRNA vs. scrambled siRNA. D and E: examples of original fluorescent images of TRPV1 labeling from a rat treated with MA after intrathecal injection of scrambled siRNA (D) or TRPV1 siRNA (E). Arrows in D and E indicate examples of single-labeled TRPV1 neurons (bright green). Scale bar in E represents 400 µm and is applied for D and E.