Fig. 7.

Western blots showing epithelial sodium channel alpha-subunit (αENaC) and mineralocorticoid receptor (MR) protein expression in mouse (m)CCD cells treated with aldosterone for 4 days (n = 3 per group). The experiment was performed in medium without serum and dexamethasone. The αENaC subunit was highly upregulated in response to aldosterone (top), whereas MR was markedly downregulated (bottom panel, lower band). The antibody against MR also recognized a higher MW band in the aldosterone-treated samples (bottom panel, upper band). ***P < 0.001 and ****P < 0.0001.