Fig. 2.

Hypoxia and H₂O₂ both increase PLCγ1 activity and inositol monophosphate-1 (IP₁) production in PASMCs. A: Western blots of PLCγ1 phosphorylated at tyrosine 783 (pTyr-783) and total PLCγ1 in PASMCs exposed to normoxia, hypoxia, untreated control, and H₂O₂ (500 µM, 10 min). B: bar graph summarizes the ratio of pTyr-783 expression level relative to total PLCγ1 protein expression level from three separate experiments. *P < 0.05 compared with respective control. C: IP₁ production in cells untreated and treated with H₂O₂ in various concentrations for 10 min. Data were obtained from three separate experiments. *P < 0.05 compared with untreated group.