Fig. 3.

PLCγ1 knock-down (KD) abolishes hypoxia- and H₂O₂-induced increase in IP₁ production in pulmonary artery smooth muscle cells PASMCs. A: summary of the hypoxia-induced IP₁ production in PASMCs. Cells were uninfected (control) or infected with lentiviral particles containing nonsilencing (NS) short hairpin (sh)RNAs or shRNAs for PLCγ1. Data were obtained from three different experiments. *P < 0.05 compared with respective normoxia group. #P < 0.05 compared with normoxia. ^ωP < 0.05 compared with normoxia. B: H₂O₂ (500 µM) induced IP₁ production in PASMCs infected with NS shRNA or PLCγ1 shRNAs. Data were obtained from three different experiments *P < 0.05 compared with NS shRNA. #P < 0.05 compared with NS shRNA.