Fig. 2.

Reactive oxygen species (ROS) and actin polymerization contribute to chronic hypoxia (CH)-induced basal arterial constriction. A: basal constriction (percentage of passive internal diameter achieved by removal of extracellular Ca²⁺) as a function of intraluminal pressure in CH arteries pretreated with tiron (10 mM). n = 7–8/group. *P < 0.05 vs. CH vehicle. CH vehicle data are from Fig. 1A. B: basal constriction in CH vessels pretreated with actin polymerization inhibitors latrunculin B (LatB; 300 nM) or cytochalasin B (CytB; 10 μM). All experiments were conducted using endothelium-disrupted arteries. n = 5–7/group. *P < 0.05, CH + LatB vs. CH vehicle; #P < 0.05, CH + CytB vs. CH vehicle.