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Endothelin-1 (ET-1) stimulates actin polymerization in pulmonary arteries from chronic hypoxia (CH) rats. A: representative fluorescence images of DNaseI 488 (green; G-actin), phalloidin 555 (red; F-actin), and TO-PRO (blue; nuclei) in pulmonary arteries from control and CH rats treated with ET-1 (10 nM) or vehicle. Drawings on the right indicate artery orientation. B: fluorescence intensity was quantified to determine F-actin-to-G-actin ratios. n = 4/group. *P < 0.05 vs. CH vehicle.
