Fig. 2.

Absence of telomerase reverse transcriptase (TERT) decreases endothelial nitric oxide synthase (eNOS) protein levels and increases mitochondrial reactive oxygen species. A: protein levels of phosphorylated eNOS (P-eNOS) and total eNOS (T-eNOS) were evaluated via Western blot analysis. T-eNOS and P-eNOS levels in mesenteric arteries (MAs) from wild-type (WT), TERT knockout (KO), and TERT transgenic (Tg) mice are shown. n = 6 mice/group. *P < 0.05. B and C: change in Mito Peroxy Yellow 1 (mitoPY1) fluorescence in WT and TERT KO mice in response to elevations in flow and in the presence or absence of polyethylene glycol-catalase (PEG-Cat). Values are means ± SD; n = 5 mice/group. *P < 0.05 vs. WT mice by t-test and one-way ANOVA with a post hoc Tukey test, respectively.