A. Time course of BCR-ABL1 transcript levels (log
scale), cytogenetic t(9;22) results (Cyto), JAK2 V617F
status, treatment, peripheral blood counts, and bone marrow morphology, Nil
= nilotinib. B. Bone marrow core biopsy at initial
diagnosis (month 0) showing features of chronic myeloid leukemia and
myelofibrosis. Hypercellular (top left), mixed population of small hypolobated
megakaryocytes and larger forms with atypical morphology (bulbous "cloud-like"
nuclei, hyperlobation) with some clustering (top right, lower). C.
Bone marrow core biopsy following tyrosine kinase inhibitor therapy showing
predominant features of myelofibrosis (month 31). Hypercellular (top left),
marked granulocytic hyperplasia, increased atypical megakaryocytes with variably
bulbous "cloud-like" morphology (top right), many intrasinusoidal megakaryocytes
(bottom left), increased reticulin fibrosis, grade 2/3 (bottom right).
D. Bone marrow core biopsy following tyrosine kinase inhibitor
and hydroxyurea therapy (month 65). Hypocellular (~10%) (top),
osteosclerosis and residual reticulin fibrosis (bottom).