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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Hepatol. 2018 Feb 16;69(1):142–153. doi: 10.1016/j.jhep.2018.02.005

Fig. 3. Binge alcohol exposure increased endotoxemia, oxidized proteins and apoptosis marker proteins with decreased TJ and AJ proteins in WT compared to Cyp2e1-null mice.

Fig. 3

The levels of (A) plasma endotoxin, (B) fecal albumin, (C) E. coli mRNA transcripts in the liver extracts assessed by real-time RT-PCR, (D) oxidized intestinal proteins (upper panel) or negative control (lower panel), (E) gut TJ proteins, (F) AJ and associated proteins, (G) gut p-JNK, cleaved caspase-3, Bax and a loading control β-actin for the indicated mouse groups (n≥8/group) are presented. Each lane indicates the mixture proteins combined from 4 different mice randomly selected within the same group. (H) Representative TUNEL staining for apoptotic enterocytes (arrows) in the indicated mouse groups. Data represent means ± SD where statistical significance was determined using two-tailed t-test: **P<0.01

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