Impacts of polymer architecture on transfection of pmaxGFP plasmid to Jurkat T cell line. Toxicity and transfection efficiency of cationic polymers with varying core shape and branch length (a & b) or core size and branch length (c & d). Transfection efficiencies are expressed as percentage of GFP-positive cells. Data are shown as mean ± SD (n=3; 1-way ANOVA with (a, c & d) Dunnett’s or (b) Tukey’s multiple comparisons, * p<0.05, ** p<0.01, *** p<0.001, ****p<0.0001).