Skip to main content
. Author manuscript; available in PMC: 2018 Dec 4.
Published in final edited form as: Nat Chem Biol. 2018 Jun 4;14(7):730–737. doi: 10.1038/s41589-018-0061-0

Figure 3. Functional separation of CoA activation in PTM and PTN biosynthesis.

Figure 3

a, PtmA1 and PtmA2 independently catalyze adenylation and thioesterification, respectively, at the C-19 carboxylic acid of PTM and PTN intermediates. b, HPLC chromatograms of thioesterification reactions catalyzed by the non-adenylate-forming acyl-CoA ligase PtmA2. Shown are boiled and enzymatic reactions with PtmA2 and overlaid standards of 6, 7, 9, and 10 (stds). Chromatograms are representative examples of n>3 independent experiments.