Fig. 3.

HO-1 induction inhibits chemokine expression. Wild-type (WT) Caco-2 intestinal epithelial cells (IECs) were treated with 50 μM of CoPP or vehicle overnight, and HMOX1 expression was measured by qPCR (a) and Western blot (b). c–e After vehicle or CoPP treatment, WT Caco-2 cells were treated with 10 ng/mL of IL-1β for 2 and 4 h, and chemokine expression assessed by qPCR. Combined results from 3 experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 compared to untreated vehicle control or other comparisons shown on the graph.