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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Immunol. 2018 May 14;201(1):157–166. doi: 10.4049/jimmunol.1701501

Fig. 5. Neuron-specific HuR-deficient mice display a molecular signature consistent with ALS.

Fig. 5

A. Lysates of NSC-34 cells were subjected to RNA immunoprecipitation with anti-HuR or anti-IgG antibody, followed by RT-PCR analysis of Ier2 mRNA. Error bars represent s.d. of biological replicates (N=15 mice for both KO and WT). ***p<0.001, by two-tailed unpaired Student’s t test. B. Whole brain extracts from experimental neuron-specific HuR-deficient and control mice as described in Fig. 1A were analyzed by Western blotting with the indicated antibodies. Western blots were quantified by densitometry using ImageJ, error bars represent s.d. of biological replicates (N=15 mice for both KO and WT), ***P<0.001 by two-tailed Student’s t test. C. Real-time PCR analysis of TDP-43 and FUS mRNA in RNA samples from whole brain tissue of experimental neuron-specific HuR-deficient and control mice. Error bars represent s.d. of biological replicates (N=15 mice for both KO and WT). ***p<0.001, by two-tailed unpaired Student’s t test. D–E. The representative confocal images of either NeuN (green) and TDP-43 (red) (D) or ChaT (green) and phospho-TDP-43 (red) (E) double staining in brain sections of experimental neuron-specific HuR-deficient and control mice. Nuclei were stained with DAPI (blue).