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. 2018 Mar 10;6(1):79–96. doi: 10.1016/j.jcmgh.2018.02.007

Figure 3.

Figure 3

mAb clones F5C12 and E5D10 show distinct in vivo staining patterns along the proximal-distal axis of the intestinal epithelium. (A) Low-magnification and (B) high-magnification images of acetone-fixed frozen mouse intestinal tissue sections stained with mAb clone F5C12 (red) within the proximal, middle, or distal small intestine and colon. (C) Low-magnification acetone-fixed frozen mouse small intestinal tissue sections stained with mAb clone E5D10 (red) within the proximal, middle, or distal small intestine and colon. White dashed lines represent the epithelial-mesenchymal boundary. Fluorescent images were captured on a Leica DMR upright microscope. Data are representative of N = 5 total mice. Scale bar: 25 μm.