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. 2018 Mar 10;6(1):79–96. doi: 10.1016/j.jcmgh.2018.02.007

Figure 6.

Figure 6

FACS gating scheme for mAbs E5D10/F5C12 in combination. (A–D) Gating strategy used to select (from left to right): cells, singlets, and live epithelial cell fractions. Data are representative of N = 12 independent experiments from N = 24 mice. (E) Left: FACS plot of intestinal epithelial cells from Lgr5-GFP reporter mice stained for mAbs E5D10 and F5C12. Inset: GFP-negative control plot. N = 6 independent experiments, N = 12 total mice. Right: FACS analysis of Lgr5GFP-hi cells and location within mAb gates. (F) Left: FACS plot of intestinal epithelial cells from Bmi1-GFP reporter mice stained for mAbs E5D10 and F5C12. Right: FACS analysis of Bmi1GFP cells and location within mAb gates. Inset: GFP-negative control plot. N = 6 independent experiments, N = 12 total mice. FACS analyses were performed on a BD Influx cell sorter at the OHSU Flow Cytometry Core Facility. FSC, forward-scattered light; SSC, side-scattered light.